Coding

Part:BBa_K187002:Design

Designed by: Team BioBytes   Group: iGEM09_Alberta   (2009-10-15)

HemF in pAB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal PstI site found at 916
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 916
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal PstI site found at 916
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal PstI site found at 916
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that the HemF gene in this plasmid starts at the start codon and ends at the stop codon. pAB does not include a promoter or terminator. pAB does include an RBS consensus sequence positioned 8 bp upstream of the ATG. We recommend that to express HemF, you use the Biobytes assembly method together with the promoter and terminator parts we have submited in pAB and pBA to assemble promoters and terminators onto HemF. As there is a range of promoters to chose from, this allows rapid manipulation of gene expression level. Using the Biobytes method, several DNA segments can be combined in just 20min per segment.

Source

MG1655 E.coli genomic DNA was the template for hemF amplification. pAB is entered as K187000.

References